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Malvern Panalytical
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New England Biolabs
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Mitochon Pharmaceuticals
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DIAGENODE DIAGNOSTICS
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Johns Hopkins HealthCare
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Illumina Inc
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Illumina Inc
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Illumina Inc
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Illumina Inc
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TaKaRa
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Lexogen GmbH
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Image Search Results
Journal: Frontiers in Pharmacology
Article Title: Binase Immobilized on Halloysite Nanotubes Exerts Enhanced Cytotoxicity toward Human Colon Adenocarcinoma Cells
doi: 10.3389/fphar.2017.00631
Figure Lengend Snippet: The scheme of the experimental work. (A) selection of conditions for loading of antitumor binase on halloysite nanotubes (HNTs) using solutions with different pH (NaAc, 10 mM Na-acetate buffer, pH 5.0; H 2 O, water of Milli-Q grade, pH 5.5; NaP, 10 mM Na-phosphate buffer, pH, 7.0; Tris-HCl, 0.25M Tris-HCl buffer pH 8.5); (B) optimal binase release from HNTs at pH 7.0. Due to the absence of difference in the quantity of released enzyme between sonicated and unsonicated samples the sonication step was excluded; (C) treatment of human colon adenocarcinoma cells Colo 320 with binase-loaded HNTs.
Article Snippet: The zeta-potential of
Techniques: Selection, Sonication
Journal: Frontiers in Pharmacology
Article Title: Binase Immobilized on Halloysite Nanotubes Exerts Enhanced Cytotoxicity toward Human Colon Adenocarcinoma Cells
doi: 10.3389/fphar.2017.00631
Figure Lengend Snippet: Characterization of binase loading on and release from HNTs. (A) The quantity of binase loaded onto HNTs at various pH (NaAc, 10 mM Na-acetate buffer, pH 5.0; H 2 O, water of Milli-Q grade, pH 5.5; NaP, 10 mM Na-phosphate buffer, pH, 7.0; Tris-HCl, 0.25M Tris-HCl buffer pH 8.5). (B) Zeta-potential of HNTs, binase-loaded HNTs and binase-loaded HNTs coated with dextrin. (C) Time-dependent release of binase from HNTs at neutral pH with and without sonication. Initial enzyme concentration in solutions was 1 mg/ml. The catalytic activity of binase in each solution is taken for 100%. Data represent mean ± SEM of three independent experiments; ∗ P < 0.05, ∗∗ P < 0.01 vs. control, ns, non-significant.
Article Snippet: The zeta-potential of
Techniques: Zeta Potential Analyzer, Sonication, Concentration Assay, Activity Assay, Control
Journal: Frontiers in Pharmacology
Article Title: Binase Immobilized on Halloysite Nanotubes Exerts Enhanced Cytotoxicity toward Human Colon Adenocarcinoma Cells
doi: 10.3389/fphar.2017.00631
Figure Lengend Snippet: Electron microscopy images of HNTs. SEM image of pure HNTs (A) , TEM image of pristine halloysite (B) and binase-loaded HNTs (C) . Arrows indicate the lumen of pristine nanotubes (B) and nanotubes loaded with binase (C) . Scale bar in (A) is 1 μm, in (B,C) is 100 nm.
Article Snippet: The zeta-potential of
Techniques: Electron Microscopy
Journal: Frontiers in Pharmacology
Article Title: Binase Immobilized on Halloysite Nanotubes Exerts Enhanced Cytotoxicity toward Human Colon Adenocarcinoma Cells
doi: 10.3389/fphar.2017.00631
Figure Lengend Snippet: Cytotoxicity of binase-loaded HNTs toward human colon adenocarcinoma Colo320 cells. Viability of cells in the absence of studied agents was taken as 100%. Data represent mean ± SEM of three independent experiments; ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001 vs. control, ns, non-significant.
Article Snippet: The zeta-potential of
Techniques: Control
Journal: Frontiers in Pharmacology
Article Title: Binase Immobilized on Halloysite Nanotubes Exerts Enhanced Cytotoxicity toward Human Colon Adenocarcinoma Cells
doi: 10.3389/fphar.2017.00631
Figure Lengend Snippet: Fluorescence microscopy of Colo 320 cells after 24 h of incubation (A) and simultaneous treatment by binase (B) , HNTs (C) , and HNTs loaded with binase (D) . Cells were stained with 3,3′-dihexyloxacarbocyanine iodide (DiOC 6 ) and propidium iodide, viable cells show green fluorescence, dead cells have red emission color. The number of viable cells is expressed as a percentage of the total cell number. 100 000 cells in each variant were taken for 100%. Scale bar is 50 μm.
Article Snippet: The zeta-potential of
Techniques: Fluorescence, Microscopy, Incubation, Staining, Variant Assay
Journal: Frontiers in Pharmacology
Article Title: Binase Immobilized on Halloysite Nanotubes Exerts Enhanced Cytotoxicity toward Human Colon Adenocarcinoma Cells
doi: 10.3389/fphar.2017.00631
Figure Lengend Snippet: Microscopic visualization of Colo 320 cells after 24 h of incubation with 100 μg/ml HNTs. Dark-field image of cells (left) , fluorescent image (middle) , merge image (right) . Cells nuclei are stained with DAPI.
Article Snippet: The zeta-potential of
Techniques: Incubation, Staining